CIVETAN   23983
CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Unidad Ejecutora - UE
artículos
Título:
Bovine Leukemia Virus Presence in Breast Tissue of Argentinian Females and Its Association With Cell Proliferation and Prognosis Markers
Autor/es:
NIETO FARIAS, MARIA VICTORIA; BUEHRING, GC; MARTÍNEZ-CUESTA, LUCÍA; DOLCINI, GUILLERMINA LAURA; MARTÍNEZ-CUESTA, LUCÍA; DOLCINI, GUILLERMINA LAURA; LENDEZ, PAMELA ANAHI; SHEN H; CERIANI, M.C; LENDEZ, PAMELA ANAHI; SHEN H; CERIANI, M.C; NIETO FARIAS, MARIA VICTORIA; BUEHRING, GC
Revista:
Multidisciplinary Cancer Investigation
Editorial:
ACECR
Referencias:
Lugar: Teheran; Año: 2018 vol. 4 p. 16 - 24
ISSN:
2476-4922
Resumen:
Introduction: Bovine leukemia virus (BLV) causes enzootic bovine leukemia, and is closely related to human T-lymphotropic virus type 1. It expresses microRNAs of unknown function and codes Tax, the protein that mediates malignant transformation. BLV is capable of infecting B- and T-lymphocytes, endothelial cells, and mammary epithelial cells of cattle. Several studies demonstrated the presence of BLV DNA in human tissue, and it is significantly associated with breast cancer in case-control studies using in situ Polymerase Chain Reaction (PCR), a highly sensitive and specific technique. The current study was the first report of BLV DNA detection by in situ PCR in tissue from Argentinian females with a diagnosis of breast cancer.Methods: In situ PCR was done to detect BLV DNA in 85 human FFPE breast cancer tissue samples. The association of BLV DNA and expression of Ki67 and Her-2 was assessed.Results: BLV DNA presence could be determined in 22.6% of the analyzed samples, and its presence was associated with an increase of the expression and prognostic markers Ki67 (P=0.009) and HER-2 (P=0.044) determined by conventional immunohistochemistry. No statistical significance was observed between the presence or absence of hormonal receptors and the presence of BLV DNA.Conclusions: The obtained results support the idea that BLV might play a role in malignant tissue transformation.