CIVETAN   23983
CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Unidad Ejecutora - UE
artículos
Título:
Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV)
Autor/es:
NIETO FARIAS, MARIA VICTORIA; PÉREZ, SANDRA ELIZABETH; MARTINEZ CUESTA, LUCIA; BARONE, LUCAS; CERIANI, MARIA CAROLINA; MARTINEZ CUESTA, LUCIA; BARONE, LUCAS; LENDEZ, PAMELA ANAHI; CERIANI, MARIA CAROLINA; DOLCINI, GUILLERMINA LAURA; LENDEZ, PAMELA ANAHI; NIETO FARIAS, MARIA VICTORIA; DOLCINI, GUILLERMINA LAURA; PÉREZ, SANDRA ELIZABETH
Revista:
VIRUS RESEARCH
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2018 vol. 256 p. 11 - 16
ISSN:
0168-1702
Resumen:
Bovine leukemia virus (BLV) is a retrovirus that affects cattle causing a lymphoproliferative disease. BLV infectionhas been associated with misbalance of the immune response causing a higher incidence of other infections.Mastitis is one of the most important conditions that affect milk production in cattle. The aim of thisstudy was to stably infect a bovine mammary epithelial cell line (MAC-T). MAC-T cell line was successfullyinfected with BLV and the infection was confirmed by nested PCR, qPCR, immunocytochemistry, western blotand transmission electron microscopy. This is the first report of a bovine mammary epithelial cell line stablyinfected with BLV. This new cell line could be used as an in vitro model to study the effect of BLV on the immuneresponse in the mammary gland and the relationship with other agents causing mastitis.