Different SNPs in Fasciola hepatica P-glycoprotein from diverse Latin American populations are not associated with Triclabendazole resistance

FERNÁNDEZ, SILVINA; SCARCELLA, SILVANA; SOLANA, HUGO; RADIO, SANTIAGO; SOLANA, MARÍA VICTORIA; TORT, JOSÉ F.; SMIRCICH, PABLO; DOMÍNGUEZ, MARÍA FERNANDA

MOLECULAR AND BIOCHEMICAL PARASITOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2018

0166-6851

The use of Triclabendazole for controlling fasciolosisis compromised by increased drug resistance affecting livestock and humans. Althoughthe mode of action of TCBZ is still unknown, putative candidates and markers of resistancehave been advanced. A single nucleotide polymorphism (T687G) in F. hepaticaPGP was proposed as marker of resistance in a small scale study of Europeansusceptible and resistant flukes, but the association was not found in Australian samples. TheT687G SNP was absent in more than 40 samples from 2 TCBZ-resistant and 3susceptible isolates across Latin America  here analyzed. While the American samples showed morevariable SNPs than the previous ones, none of the SNPs detected showed amarked association with resistance. Analyzing the 42 kb of the FhPGP gene based on RNAseqdata highlights that the variation has been underestimated, suggesting that moredetailed efforts are needed in order to identify markers of resistance.