Molecular analysis of dairy cows from Pasto, Nariño fragment of bovine leukemia virus env gen by nested-PCR in dai

CERIANI CAROLINA; BENAVIDES BIBIANA; MUÑOZ SEBASTIAN

Revista de Medicina Veterinaria

Dialnet

Lugar: Bogota; Año: 2017 vol. 33 p. 67 - 75

0122-9354

Introduction: Routine diagnosis for bovine leukemia is performed using indirect serologictests, although it is recommended to use polymerase chain reaction (PCR), which allows areliable diagnosis in early stages and in young animals. Moreover, by amplifying the DNA,it is possible to sequence fragments of the virus, which allows to identify genotypes and toconstruct phylogenetic trees. Objective: To analyze a fragment of the env gene of bovine leukemiavirus (BLV) isolated from indirect ELISA-positive animals in dairy farms in the municipalityof Pasto (Nariño). Materials and methods: Once the presence of BLV was establishedin 48 animals over two years old in seven dairy farms in the municipality of Pasto byindirect ELISA test, a nested PCR test was performed to confirm diagnosis and to sequencea fragment of the env gene in positive animals and their daughters. The sequences obtainedwere compared with the seven genotypes described worldwide by MEGA 6 program. Results:The sequences of the compared fragments do not differ from those described; butthey allow their grouping into different clusters according to their similarity. The genotypesfound in these farms correspond to genotype 1 and 2 described in the literature. Conclusions:Only one genotype was found on farms with proper recovery and adequate biosecuritymeasures, and on farms with less control in management and recovery practices, bothgenotypes described for the region were eviden