MLVA typing of Mycoplasma hyopneumoniae bacterins and field strains

TAMIOZZO, P.J.; ZAMORA, R.; LUCCHESI P.M.A.; ESTANGUET, A.; PARADA, J.; CARRANZA, A.; CAMACHO, P.; AMBROGI, A.

Veterinary Record Open

British Veterinary Association

Año: 2015 vol. 2015 p. 1 - 5

2052-6113

Because of the lack of information about both the genetic characteristics of M. hyopneumoniae commercial vaccines and their relationship with field strains, we attempted to identify genetic subtypes of some M. hyopneumoniae bacterins and to compare them with M. hyopneumoniae field strains. Six commercial M. hyopneumoniae bacterins and 28 broncho-alveolar lavages from pigs at slaughter from three herds were analyzed by Multiple-Locus Variable-Number of Tandem-Repeat Analysis (MLVA) on p146R1, p146R3, H4, H5 and p95 loci. The results obtained showed the presence of more than one M. hyopneumoniae genotype in some pigs and also in one of the bacterins analyzed. It is also worth noting that MLVA typing allowed the distinction not only among circulating field strains but also when comparing them against vaccine strains, which knowing the relatedness among them, could be useful in the research of the efficacy of the vaccines.