ACTIVITY GLUTATHIONE S-TRANSFERASE IN HELMINTHS EXPOSED ALBENDAZOLE

FERNÁNDEZ V.; CADENAZZI G.; SCARCELLA S.; LAMENZA P.; SOLANA H.

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2013 vol. 37 p. 13 - 34

0327-9545

The worms are a major medical problem in both pet health andhumans being the key tool to combat chemical control. The AscariasisFasciolosis and are a major cause of losses in livestockproduction and its control is mainly based on the use of albendazole(ABZ). All helminths have different biochemical mechanisms fordetoxification. This study aimed to evaluate a model ex vivo effectof ABZ on the activity of the detoxificative enzyme  of Phase II,glutathione S-transferase (GST) in Fasciola hepatica and Ascarissuum. Five (5) Ascaris and flukes were placed immediately collectedwith varying concentrations of ABZ in PBS medium at 37ºC. After4 h, were processed to obtain high speed supernatant evaluatedGST activity in the cytosol. This activity was inhibited in a concentrationdependent manner. So, 10 nmoles of ABZ caused a decreasein GST activity in Fasciola (40%) and  Ascaris (25%). With 20 nmolof ABZ decreased 64% in Fasciola and 40% in Ascaris . These preliminaryresults contribute to the understanding of the response of the  helminth and understanding the effect of ABZ effect.