CIVETAN   23983
CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Unidad Ejecutora - UE
artículos
Título:
Optimization of production of chlamydospores of the nematode-trapping fungus Duddingtonia flagrans in solid culture media
Autor/es:
SAGÜÉS, M.F, FUSÉ, L.A., IGLESIAS, L.E., MORENO, F.C., SAUMELL, C.A.
Revista:
PARASITOLOGY RESEARCH
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2013 vol. 112 p. 1047 - 1051
ISSN:
0932-0113
Resumen:
The large-scale production of nematophagous fungias agents of biological control is one of the main challengesto be commercially used. In order to improve growth ofmicroorganism in a culture medium, the addition of growthinducer is common. At the moment, the action of their additionin the mycelia growth and sporulation rate of nematophagousfungi is not known. The purpose of this trial was toevaluate the sporulation rate of Duddingtonia flagrans byadding two growth inducers, meso-inositol and Tween 80,both at 0.5 % in a traditional culture medium Sabouraudglucose agar (SGA) and also in a traditional culture mediumenriched with wheat flour and milk powder. From a traditionalsterile culture of D. flagrans, four groups were made: SGA;Sabouraud glucose agar?meso-inositol 0.5 %; Sabouraud glucoseagar?Tween 80 0.5 %; and Sabouraud glucose agarenriched(SGA-E). These media were placed at a constanttemperature of 27 °C for 4 weeks. Following this, chlamydosporeswere gently rinsed off with sterile water and countedusing a Neubauer haematocytometer to estimate the numberof chlamydospores per millilitre of water. The addition ofmeso-inositol 0.5 % to SGA promoted a significant increase(p<0.05) in chlamydospore production obtaining an averageof 51,715,000 chlamydospores per Petri dish. The highestchlamydospore concentration was observed in the SGA-E incomparison with SGA (p<0.01) obtaining an average of208,760,000 chlamydospores. The aim of this study was toobtain basic knowledge regarding the effect of enriched culturemedium and growth-inducing meso-inositol and Tween80 on mycelial growth and production of chlamydospores.