CIVETAN   23983
CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Unidad Ejecutora - UE
artículos
Título:
IN VITRO PENETRATION OF FOSFOMYCIN IN RESPIRATORY CELLS
Autor/es:
PÉREZ, D. S., SORACI A. L., TAPIA M. O
Revista:
THE PIG JOURNAL
Editorial:
UK PIG VETERINARY SOCIETY
Referencias:
Lugar: Thirsk; Año: 2012 vol. 67 p. 43 - 53
ISSN:
1352-9749
Resumen:
Infectious diseases of the respiratory systemare important in intensive swine production. Fosfomycin is a widely used antibiotic for the treatment of swine lung infections.The aim of this research was to study the penetration of fosfomycin in an in vitro model of respiratory cells (HEp-2 cells). Cell cultures were subjected to four treatments: a) negative control; b) disodiumfosfomycin(280μg/mL); c) disodiumfosfomycin (130μg/mL) and d) calciumfosfomycin (130μg/mL). Intracellular concentrations of fosfomycin were analysed by high-performance liquid chromatography tandemmass spectrometry (HPLCMS/MS).Concentrations in HEp-2 cells incubatedwith 280μg/mL of disodiumfosfomycin ranged from 0.74 to 2.79μg/mL (Tmax: 12 hours). When incubated with the same formulation of fosfomycin at a concentration of 130μg/mL, intracellular concentrations ranged between 0.31 and 1.60μg/mL (Tmax: 12 hours). Calcium fosfomycin reached intracellular concentrations that varied between 0.46 and 1.11μg/mL (Tmax: 8 hours). Fosfomycin concentrations exceeded the MIC90 for themost important pathogens in swine respiratory infections (Streptococcus spp.;0.25μg/mL).Therefore, it is apparent that fosfomycin is an alternative for the treatment of intracellular respiratory infections in pigs.