CIVETAN   23983
CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Unidad Ejecutora - UE
artículos
Título:
"Optimization of production of chlamydospores of the nematode-trapping fungus Duddingtonia flagrans in solid culture media"
Autor/es:
SAGÜÉS MARÍA F.; FUSÉ LUIS A.; IGLESIAS LUCÍA E.; MORENO FABIANA C.; SAUMELL CARLOS A.
Revista:
PARASITOLOGY RESEARCH
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2012
ISSN:
0932-0113
Resumen:
The large-scale production of nematophagous fungi as agents of biological control is one of the main challenges to be commercially used. In order to improve growth of microorganism in culture medium, it is common the addition of growth-inducer. At the moment, the action of their addition in the mycelia growth and sporulation rate of nematophagous fungi is not known. The purpose of this trial was to evaluate the sporulation rate of Duddingtonia flagrans by the adding of two growth-inducers, meso-inositol and tween 80, both at 0.5% in a traditional culture medium sabouraud glucose agar (SGA), and also in a traditional culture medium enriched with wheat flour and milk powder. From a traditional sterile culture of D. flagrans four groups were made: sabouraud glucose agar (SGA); sabouraud glucose agar meso-inositol 0.5 % (SGA-MI 0.5%); sabouraud glucose agar-tween 80 0.5% (SGA-TW8 0.5%) and sabouraud glucose agar - enriched (SGA-E). These media were placed at a constant temperature of 27ºC for four weeks. Following this, chlamydospores were gently rinsed off with sterile water and counted using a Neubauer haematocytometer to estimate the number of chlamydospores per ml of water. The addition of meso-inositol 0.5% to SGA promoted a significant increase (p