PERSONAL DE APOYO
Transcriptomic, proteomic and metabolomic analysis of maize responses to UV-B
VIRGINIA WALBOT, DARREN MORROW, JOHN FERNANDES, MABEL CAMPI Y PAULA CASATI
Plant Signaling and Behavior
Año: 2011 vol. 6 p. 1146 - 1153
UV-B radiation from normal solar fluence elicits physiological and developmental changes in plants under fluctuating environmental conditions. Most UV photobiology studies in plants utilize controlled greenhouse and growth chamber environments in which few conditions vary except the brief presence of UV-B radiation. Our purpose was to compare responses to UV-B in irradiated and shielded maize organs in field (natural solar plus 2x solar supplementation for defined periods) and greenhouse (2x solar supplementation only) conditions during a 4 h exposure. Three parameters were assessed?transcripts, proteins and metabolites?to determine the degree of overlap in maize responses in field and greenhouse conditions. We assessed irradiated leaves, and both shielded leaves and immature ears. After comparing transcriptome, proteome and metabolome profiles, we find there are more differences than similarities between field and greenhouse responses.