Erythropoietin and iron availability in the regulation of hepcidin in hepatic cells.

IOLSTER, JOAQUÍN; MALTANERI, ROMINA; NESSE, ALCIRA; CHAMORRO, M. EUGENIA; VITTORI, DANIELA

Evento virtual

Congreso; LXV Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2020

Sociedad Argentina de Investigación Clínica

Maintenance of systemic iron (Fe) levels undergoes regulation by the erythropoietic demand, inflammation and Fe status. Liver hepcidin (Hep), a small peptide which binds the Fe exporter ferroportin thus inducing its internalization and degradation, is a key regulatory target for Fe homeostasis. We examined the ability of erythropoietin (Epo) to regulate Hep mRNA expression (real-time PCR) in the human hepatic cell line HepG2. Control (C) Hep levels were significantly reduced by Epo (160 ng/mL, 6 h), while its non-erythropoietic, carbamylated derivative cEpo failed to exert this effect (C: 1, *Epo: 0.4±0.2, cEpo: 0.9±0.1; *P