Decidual factors and vasoactive intestinal peptie guide monocytes and decidual macrophages to higher migration, efferocytosis and wound healing in term human pregnancy

FERNÁNDEZ, L.; CORREA-SILVA, S.; HAUK, V.; PEREZ LEIRÓS, C.; GRASSO, E.*; WEINGRILL-BARBANO, R.; ABASOLO, J.; BEVILAQCUA, E.; PAPARINI, D.*; MERECH, FATIMA; IZBIZKY, G.; RAMHORST, R.

Congreso; SAI-SAIC-SAFIS 2020; 2020

The recruitment and differentiation of monocytes (Mo) at the decidua are critical events that determine the outcome of pregnancy. Their functional plasticity limits the extent of injury after implantation and promotes tissue homeostasis maintenance. The vasoactive intestinal peptide (VIP) is an immunoregulatory peptide synthesized by trophoblast and decidual cells that promotes trophoblast (Tb) invasion, vascular remodelling and functional shaping of decidual macrophages (dMA) in first trimester placenta. Here we studied the role of VIP in human term placenta as a regulator of Mo and dMA function. Peripheral blood monocytes were isolated from non-pregnant(NON-P) and pregnant (P) volunteers and normal placental samples were obtained at term. Mo were treated in vitro with VIP, or conditioned media from decidual explants (D) or decidual explantscultured with VIP [D (VIP)]. Mo or dMA isolated by positive selection with magnetic beads were used to study efferocytosis with CFSE-labelled autologous apoptotic neutrophils by flow cytometry and their wound healing capacity on human endometrial stromal cell line(HESC) monolayers. NON-P Mo presented higher percentage of efferocytosis than P(34.5±4.5 vs. 21.8±3.3; p