VIP DEFICIENCY PROMOTES AN INFLAMMATORY OVARIAN MICROENVIRONMENT IMPAIRING PREGNANCY

HAUK, VANESA; PASCUALI NATALIA; WASCHEK, JAMES; GALLINO LUCILA; GRASSO ESTEBAN; NAGUILA, ZAIRA; RAMHORST ROSANNA; MERECH, FÁTIMA; PARBOREL FERNANDA; PEREZ LEIROS CLAUDIA

Congreso; Reunion de Biociencias Virtual 2020; 2020

SAI

Background: Complex immune regulation is required to ensure a successful pregnancy outcome. Vasoactive intestinal peptide (VIP) is an immunoregulatory peptide known to induce a tolerogenic maternal response. Moreover, VIP regulates folliculogenesis and ovulation as well as ovarian steroidogenesis. VIP KO mice are known to be subfertile, so we aimed to better understand how VIP deficiency affects ovarian physiology and immune homeostasis.Methods: Animals: VIP Knockout KO (-/-), deficient HT (+/-) and wild type WT (+/+) mice in estrus (defined by vaginal lavage observation) were studied at 3 or 8 months of age. Serum hormones were measured with immulite Xpi Siemens platform. Ovaries were examined after histological staining, hormones quantified using RIA and IL-1 secretion by ELISA. Results: Young KO mice showed cycling disorders accompanied by an altered P4/E2 ratio in serum and ovary in the day of estrus as compared to WT mice of the same age. We also found histological differences between the ovaries, with an increase in secondary follicles, less corpora lutea, and more atretic follicles (p