IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Reticular Stress through ATF6α pathway induce an inflammatory response: potential regulation by miRNAs
Autor/es:
E.SOCZEWSKI, J. MURRIETA-COXCA, L. MIRANDA , R.GUTIÉRREZ-SAMUDIO, L. GALLINO , E. GRASSO, L. FERNÁNDEZ, S. GORI, M.MARTI, D.MORALES-PRIETO, R. FAVARO, C. PÉREZ LEIRÓS, U.MARKERT,.R. RAMHORST
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Anual Conjunta SAIC-SAI-SAFIS; 2020
Resumen:
During decidualization, endometrial stromal cells undergo endoplasmic reticulum stress (RS) and unfolded protein response (UPR), which will allow them to expand their endoplasmic reticulum with the corresponding machinery for protein folding. These processes are directed by miRNAs that regulate the expression or stability of their transcription factors. Here we focus on the role of RS and UPR during the decidualization to induce a physiological sterile inflammatory response and whether might be regulate by miRNAs. We used an in vitro model of decidualization represented by human telomerase-immortalized endometrial stromal cell line St-T1b, treated with 8-Br-cAMP (0.5 mM) during 5 days, or Thapsigargin (Tg, a RS-inducer 1 µg/ml); and endometrial biopsies from patients with recurrent spontaneous abortions (RSA) and recurrent in vitro fertilization failures (RIF).We evaluated the expression of the RS-sensor ATF6 and the UPR marker, CHOP. Both markers increased in decidualized cells, and Tg induce even higher levels in comparison with non-decidualized cells (p