High Molecular Weight Chitosan as a functional biomaterial. Properties and cytotoxic evaluation

AGUSTINA ALAIMO; OSCAR E PÉREZ; MARIANA CAROLINA DISANTO; REGINA DE MATTEO; ANA PAULA DOMINGUEZ RUBIO

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Congreso; LatinXChem - Chemistry Congress; 2020

Royal Society of Chemistry, ACS chemistry, ChemNanoMat, Springer, Wiley, entre otros

Biomaterials refer to biologically derived raw materials applied primarily for their structural and biological properties. Between the major requirements, biomaterials should be biocompatible, non-immunogenic and biodegradable. In this work, we characterized a new High Molecular Weight Chitosan (H-CS) obtained from chitin extracted from the residues of Pleoticus muelleri shrimps, the main crustacean exploited in Mar del Plata, Argentine maritime platform of the Atlantic Ocean. This H-CS could have practical implications in food, cosmetic, nutraceuticals and clinical therapies. We assessed the biocompatibility of H-CS from Argentinean shrimp?s wastes in prokaryotic and eukaryotic cell models. Ultrastructure of H-CS was analysed by SEM and TEM. To test biocompatibility, in vitro studies were performed in prokaryotic (Lactobacillus casei BL 23) and eukaryotic (Caco-2, ARPE-19, EA.hy926 and 3T3-L1) culture cells. We found that retinal pigment epithelium as well as fibroblast and endothelial cells exhibited a good cellular response reflected by biocompatibility and effective uptake of the H-CS. The proliferation effect cannot be explained just by the simple uptake into the cells. Mechanistic studies of molecular nature must be addressed to answer this question. H-CS did not evoke appreciable adverse effects on the lactic acid bacteria growth colonies. Our work also explored the effect of H-CS on human intestinal cells, being able to verify its biocompatibility. In summary, H-CS could be a suitable biopolymer to be exploited for biomedical or food industry applications.Twitter Congress - Time Zone: the Greenwich Meridian in London, England (UTC)