IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Microencapsulation of probiotic lactic acid bacteria into a matrix of Argentine chitosan. Its application as extracellular vesicles-controlled delivery device and its impact in the host digestive tract
Autor/es:
PIURI, MARIANA; D ANTONI, CECILIA; PÉREZ, OSCAR E; ANA PAULA DOMINGUEZ RUBIO
Lugar:
Quilmes, Prov de Bs As.
Reunión:
Congreso; Nanomed-ar 2020.; 2020
Institución organizadora:
Asociación Argentina de Nanomedicinas
Resumen:
The aim of this work plan is to encapsulate the probiotic Lacticaseibacillus casei BL23 into an Argentine chitosan (Q) matrix in order to increase its survival during food processing, storage and the transit through the digestive system, until reaching the gut, where they would function as extracellular vesicles (EVs)-delivery device. This strategy would protect bacteria from changes in pH, temperature and osmotic stress, as well as from bacteriophage attack during fermentation processes of food.The probiotic effect of L. casei BL23 EVs in in vitro, ex vivo and in vivo models of inflammation will be evaluated. We hypothesize that microencapsulation would increase the residence time of the bacteria in the gut and thus its probiotic effects. In addition, the mucoadhesive properties of Q would allow a sustained release of EVs. Bacterial EVs were able to pass through gut epithelial cells and reach the host bloodstream and finally its target tissues as demonstrated by Domímguez-Rubio et al 2020 .Analytical techniques such as dynamic light scattering, nanoparticle tracking analysis, Z potential determinations, electron, atomic force, fluorescence and confocal microscopy, molecular biology and immunological techniques (ELISA, flow cytometry) will be used. Dehydration in spray systems will be implemented to achieve microencapsulation.