Chitosan-based nanomedicine for ocular treatments.

CAROLINA DISANTO; GARCÍA LIÑARES, GUADALUPE; BUOSI, FLORENCIA SOLANA; DOMINGUEZ RUBIO, ANA PAULA ; OSCAR E. PÉREZ; ALAIMO, AGUSTINA; CHAUFAN, GABRIELA

Quilmes, Prov de Bs As.

Congreso; Nanomed-ar 2020.; 2020

Asociación Argentina de Nanomedicinas

Oxidative stress and inflammation play a key role in many ocular pathologies. Resveratrol (RSV), a natural polyphenol with antioxidant properties, has gained interest in the scientific community for its antioxidant and anti-inflammatory characteristics. However, its therapeutic potential is restricted by its low solubility and photosensitivity. In the present work, Chitosan (300kDa)-based nanogels (Q-NG) were synthesized by ionic gelation in order to overcome the obstacles that the RSV present.By DLS, we determined that Q-NG exhibited a monomodal size distribution around 140 nm with a ζ-potential value of 32 ± 2 mV. AFM and TEM images showed that NG exhibited a spherical morphology. RSV encapsulation efficiency was 59 ± 1%. Furthermore, encapsulated RSV was protected against UV light-induced photodegradation. Biocompatibility tests revealed that Q-NG were not cytotoxic nor immunogenic for human retinal pigment epithelial cells. Finally, once internalized in the cells, the Q-NG escape from the endo / lysosomal degradative pathway, which is crucial for efficient nanocarriers delivery systems. In conclusion, Q-NG could constitute carriers for RSV, opening the chance of its application for ophthalmic use.