DECIDUAL FACTORS AND VASOACTIVE INTES- TINAL PEPTIDE GUIDE MONOCYTES AND DECICUAL MACROPHAGES TO HIGHER MIGRATION, EFFEROCY- TOSIS AND WOUND HEALING IN TERM HUMAN PREG- NANCY

ESTEBAN GRASSO; WEINGRILL?BARBANO, RODRIGO; ABASOLO, JOSÉ I.; BEVILAQCUA, ESTELA; DANIEL PAPARINI; FATIMA MERECH; IZBIZKY, GUSTAVO; RAMHORST, ROSANNA; FERNÁNDEZ, LAURA; CORREA?SILVA, SIMONE; HAUK, VANESA; CLAUDIA PEREZ LEIROS

Congreso; Congreso de la Sociedad Argentina de Inmunología 2020; 2020

The recruitment and differentiation of monocytes (Mo) at the de-cidua are critical events that determine the outcome of pregnancy.Their functional plasticity limits the extent of injury after implantationand promotes tissue homeostasis maintenance. The vasoactive in-testinal peptide (VIP) is an immunoregulatory peptide synthesizedby trophoblast and decidual cells that promotes trophoblast (Tb)invasion, vascular remodelling and functional shaping of decidualmacrophages (dMA) in first trimester placenta. Here we studied therole of VIP in human term placenta as a regulator of Mo and dMAfunction.Peripheral blood monocytes were isolated from non-pregnant(NON-P) and pregnant (P) volunteers and normal placental sam-ples were obtained at term. Mo were treated in vitro with VIP, orconditioned media from decidual explants (D) or decidual explantscultured with VIP [D (VIP)]. Mo or dMA isolated by positive selectionwith magnetic beads were used to study efferocytosis with CFSE-la-belled autologous apoptotic neutrophils by flow cytometry and theirwound healing capacity on human endometrial stromal cell line(HESC) monolayers.NON-P Mo presented higher percentage of efferocytosis than P(34.5±4.5 vs. 21.8±3.3; p