IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
LPS FROM P. GINGIVALIS AFFECTS TROPHO- BLAST-NEUTROPHIL INTERACTION THROUGH TLR4 AND FAVORS A PROINFLAMMATORY MILIEU
Autor/es:
GUILLERMINA CALO; DANIEL PAPARINI; VANESA HAUK; BRENDA LARA; FATIMA MERECH; CLAUDIA PEREZ LEIROS; DAIANA VOTA; ROSANNA RAMHORST
Reunión:
Congreso; Congreso de la Sociedad Argentina de Investigación Clínica 2020; 2020
Resumen:
During placentation, trophoblast cells interact and secrete cytokinein order to regulate and maintain immune homeostasis. Changes ordefects in this interaction may lead to pregnancy complications. Infact neutrophil activation is associated with poor placentation andsevere pregnancy complications. Porphyromonas gingivalis (Pg)is an important pathogen of periodontal disease that has been im-plicated in adverse pregnancy outcome although the mechanismsinvolved are still unclear. Pg-LPS is the most important virulencefactor of Pg and activates both TLR4 and TLR2.The aim of this work was to evaluate the effect of conditioned mediaof trophoblast cells primed with Pg-LPS on neutrophil function.Human trophoblastic cell line Swan-71 was treated with Pg-LPS(10ng/ml) or Pg-LPS ultrapure, variant that only activates TLR4. Cy-tokine expression was evaluated by RTqPCR, flow cytomery andELISA. Peripheral blood neutrophils (Neu) and mononuclear cells(PBMCs) were purified from healthy donors and cultured with condi-tioned media from trophoblast cells (TbCM) treated or not with LPS(PgLPS-CM) or LPS ultrapure (PgLPSultra-CM). Apoptosis andreactive oxygen species (ROS) were evaluated by flow cytometry.Regulatory T cell induction was evaluated by flow cytometry.Pg-LPS ultrapure increasde the expression of TNFa, IL6 and IL1brespect to basal while Pg-LPS had no effect. In line with this result,Pg-LPS-CM had no effect on neutrophil activation whereas PgLP-Sultra-CM increased neutrophil activation with higher release ofROS and decreased apoptosis rate (p≤0.05). Neutrophils exposedto TbCM induced FOXP3 expression on CD4+ T cell after 48h of co-culture with PBMCs. This induction was diminished when neutrophilwere preconditioned with PgLPS-CM or PgLPSultra-CM (P