IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
The Aryl Hydrocarbon Receptor Inhibition Blocks Dengue and Zika Flavivirus Infection
Autor/es:
GIOVANNONI, F.; GARCÍA, C. C.; DAMONTE, E.B.; TORTI, M.F.; QUINTANA, F.
Lugar:
Baltimore, Maryland
Reunión:
Conferencia; 32nd International Conference on Antiviral Research; 2019
Resumen:
The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that has been classically associated with the clearance of xenobiotics. Recently, numerous studies have indicated that AHR is capable of modulating the immune system. Regarding to viral infections, it was shown that the stimulation of AHR not only reduces the survival of mice infected with influenza A virus, but also enhances the hepatitis C virus assembly and production in vitro. Dengue virus (DENV) belongs to the Flaviviridae family and has four serotypes (DENV-1,2,3,4) that are capable of causing illness in humans after the bite of infected mosquitoes of the genus Aedes. Nowadays, specific treatments do not exist, thus it is crucial the development of new antiviral strategies. Previous results from our group indicates that the blockade of AHR, inhibits the Zika Virus (ZIKV) replication in vitro. In the present study we evaluated the impact of the pharmacological modulation of AHR on the DENV infection in vitro. Agonists and antagonists of AHR were used to treat A549 cell cultures before the infection with DENV1-4. From the supernatants of the cultures, we determined the viral yields. We also performed, real-time RT-PCR and indirect immunofluorescence to quantify the viral genome and protein, respectively. The treatment with 20µM of the AHR antagonist CH223191 decreased the viral yield 95±4% and the protein expression in a 84,5±0,5%. The data obtained suggests the AHR signaling pathway is involved in the DENV