IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
UNDERSTANDING THE NATURAL SELECTION OF HUMAN EMBRYOS: BLASTOCYST QUALITY MODULATES THE GENERATION OF A TOLEROGENIC MICROENVIROMENT BY DECIDUALIZED CELLS
Autor/es:
LAURA FERNÁNDEZ; DANIEL PAPARINI; ESTEBAN GRASSO; MARIA SOLEDAD GORI; GUSTAVO MARTÍNEZ; ROSANNA RAMHORST; ELIZABETH SOCZEWSKI; CLAUDIA PÉREZ LEIRÓS
Lugar:
Buenos Aires
Reunión:
Congreso; International Federation of Placenta Associations 2019 (IFPA2019) - 8th Latin American Symposium on Maternal-Fetal Interaction and Placenta (VIII SLIMP); 2019
Institución organizadora:
IFPA & SLIMP
Resumen:
ObjectivesDecidualized cells sense embryo quality allowing the implantation of normal development (ND) blastocysts while preventing the invasion of impaired development (ID) ones. Understanding the molecular basis of this process may contribute to improve infertility treatments. We aim to characterize the immune microenvironment generated by decidualized cells in response to solublefactors secreted by ND and ID blastocysts.MethodsHuman endometrial stromal cell line (HESC) was in vitro decidualized with medroxiprogesterone and dbcAMP during 8 days. Then, decidualized cells (dHESC) were stimulated with blastocyst conditioned media (BCM). BCM were recovered from 5 days individually cultured blastocyst obtained from IVF or ICSI and classified as ND or ID according to morphological standards. After 24h BCM stimulation, dHESC gene expression (RT-qPCR) and protein production (flow citometry)were evaluated. Supernatants from 48h stimulation were used to test Tregs migration (transwell system) and during dendritic cells (DCs) differentiation. For statistical analysis, p