Vasoactive Intestinal Peptide induces glucose and neutral amino acid uptake through mTOR pathways in human trophoblast cells.

F.I MERECH, E. SOCZEWSKI, V. HAUK, D. PAPARINI, R. RAMHORST, C. PÉREZ LEIRÓS, D. VOTA

Buenos Aires

Congreso; International Federation of Placenta Association meeting 2019; 2019

Objective: Nutrient transport across the placenta and sensing of nutrient levels through mTOR-mediated pathways is crucial for fetal growth. We have shown that vasoactive intestinal peptide (VIP), synthesized by human trophoblast (Tb) cells, increases human Tb migration and invasion. Also, VIP has embryo trophic effect in mice. Our aim was to evaluate the effect of VIP on glucose and neutral amino acid uptake in Tb cells and the interplay between VIP and mTOR.Methods: Human trophoblast-derived cell lines Swan-71 (SW) and BeWo (BW) treated with VIP or silenced in VIP expression were used. Glucose and System A amino acid specific uptake was measured in single living cells by D-glucose fluorescent analog (2-NBDG) or the specific System A radiolabelled substrate (14C-MeAIB) incorporation respectively. GLUT and SNAT transporters, VIP and mTOR expression was evaluated by qRT-PCR or flow cytometry.Results: VIP induced a 31.2 ± 6.2% and 38.2 ± 7.4% increment of glucose uptake in SW and BW (p