VIP deficiency is associated with a immune dysregulation in uterus and consequent failure pregnancy

LUCILA GALLINO1, VANESA HAUK, SOLEDAD GORI, NATALIA PASCUALI, FERNANDA PARBORELL F ,MARCOS PALLIGAS, NORA SARACO, ESPERANZA BERENSZTEIN, JAMES WASCHEK, CLAUDIA PEREZ LEIRÓS AND ROSANNA RAMHORST

Tucumán

Congreso; Reunión Anual Sociedad Argentina de Inmunología; 2019

Sociedad Argentina de Inmunología

Background: VIP is a master regulatory peptide in the feto-maternal interface with antiinflammatory effects inducing a tolerogenic maternal response. In ovary regulates the follicle survival, the oocytes maturation and ovulation as well as steroidogenesis. Since VIP KO mice show pregnancy complications we aimed to explore alterations in the immune microenvironment in ovary and uterus that could condition embryo implantation. Methods: Animals used: VIP Knockout KO (-/-), deficient HT (+/-) and wild type WT (+/+) 3 months old in estrus. The serum was analyzed with immulite Xpi Siemens platform. The uterus and ovaries were examined after histological staining and/or RT-PCR. The estrous cycle was defined after observation of the cellular components under light microscopy. Results: After 2 weeks, we found that HT and KO mice showed cycling disorders accompanied by a different P4/E2 ratio in serum and ovary compared with WT. On the day of estrus, we found histological differences between the ovaries and uterus of WT mice vs KO mice. In the uterus, these changes were accompanied by undetectable levels of expression of Foxp3 in the KO group, higher expression of RORγt and a decrease in IL-10 (p