Effects of co-culture conditions on the metabolic transcriptomic profile of prostate cancer cells

BIZZOTTO JUAN; GUERON GERALDINE; SANCHIS PABLO; COTIGNOLA JAVIER; ANSELMINO NICOLAS; VAZQUEZ ELBA

Mar del Plata

Congreso; Reunión Anual de la Sociedad Argentina de Investigación Clínica 2019; 2019

SAIC

Prostate cancer (PCa) is the most common type of cancer diagnosed in men being bone themain site of progression. Tumor cells interact with the bone microenvironment and disrupt thetissue balance. Alterations in energy metabolism have emerged as one of the hallmarks ofcancer, evidenced by the numerous connections between signaling pathways that includeoncoproteins and key enzymes in energy metabolism. Variations in a single gene couldorchestrate a change in the metabolic fate of the cell and thus confer an adaptative advantage.In this work, we aimed at identifying cellular energetic deregulations of metastatic PCa cellsusing an in vitro co-culture transwell system, in which PCa cells (PC3) were grown alone or co-cultured with osteoblasts precursors (MC3T3 cells). RNAseq was performed on tumoral cellsand differential gene expression analysis was performed. The gene ontology (GO) analysisrevealed an enrichment of ketone and lipid metabolism in PC3 co-cultured with MC3T3compared to PC3 grown alone (p