CONDITIONED MEDIA FROM ERYTHROPOIETIN-TREATED ERYTHROID CELLS SUPPRESS HEPCIDIN IN THE HEPG2 HEPATIC CELL LINE

ÁLVAREZ, GUADALUPE; MALTANERI, ROMINA; NESSE, ALCIRA; GENOUD, VALERIA; VITTORI, DANIELA

Mar del Plata

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2019

Sociedad Argentina de Investigación Clínica

Erythropoiesis is a key regulator of Fe metabolism, as it demands a high supply of the metal for hemoglobin synthesis. In patients, erythropoietin (Epo) was found to decrease liver hepcidin (Hamp), thus allowing the release of stored Fe into the bloodstream. We previously reported a direct downregulation of Hamp by Epo in the human HepG2 cell line, as early as 6 h after exposure to the cytokine. However, other authors have postulated the existence of an erythroid regulator ?erythroferrone (ERFE)?, which is produced by erythroblasts upon Epo stimulation.To investigate the indirect activity of Epo on Hamp expression in hepatic cells, human erythroleukemia K562 cells were treated with Epo (10 U/mL, 24 h), thoroughly washed and cultured in growth medium alone for other 24 h, obtaining conditioned media (CM). HepG2 cells were exposed to the CM (6 h), and Hamp levels were quantified by real-time PCR. CM of Epo-treated K562 cells (CM-E) significantly decreased Hamp mRNA compared to CM of control K562 cells (CM-C=1.00; *CM-E=0.33±0.12; *P