IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Ocular drug delivery: nanoformulations based on Argentinian chitosan for resveratrol encapsulation
Autor/es:
CASTAÑEDA CATAÑA, MAYRA ALEJANDRA; DOMINGUEZ RUBIO, ANA PAULA; PÉREZ, OSCAR EDGARDO; BUOSI FLORENCIA; MARTÍNEZ, KARINA; ACEBEDO, SOFÍA LORENA; ALAIMO, AGUSTINA; DI SANTO, MARIANA CAROLINA; SPAGNUOLO, CARLA CECILIA
Lugar:
Ciudad Autónoma de Buenos Aires
Reunión:
Jornada; IX Jornadas de Jóvenes Investigadores; 2019
Institución organizadora:
Facultad de Ciencias Veterinarias, Universidad de Buenos Aires
Resumen:
The retinal tissue has the highest oxygen uptake and glucose oxidation relative to any othertissue, which makes it more susceptible to oxidative stress. Constant light irradiation,atmospheric oxygen and environmental chemicals contribute to oxidative stress as the keyfactor for the ocular degenerative diseases. Whenever the antioxidant defense systems aredepleted or not functional, vision impairment might be observed. For this reason antioxida ntsare fundamental agents in prophylactic forms of oxidative diseases. However, considering itssensitive structure, nanotechnology becomes an option in antioxidant protection, stabilit y,bioavailability, and therapeutic efficacy. Therefore, the aim of this work is to developnanoparticles (NPs) as carriers of antioxidants for the ocular diseases prevention.Particularly, there is an increased interest in the therapeutic effects of Resveratrol (RSV).RSV is a naturally occurring polyphenolic compound, mainly found in black grapes andpeanuts. Previous studies have demonstrated the various beneficial effects of RSV on humanhealth, including its neuroprotection, anti-inflammatory action, potent antioxidant activit yand anti-aging effects. Chitosan (CHI) is a naturally occurring and abundantly availablepolysaccharide extensively studied for drug delivery systems due to the biological propertiessuch as its relative non toxicity, biocompatibility, biodegradability, cationic properties andmucoadhesive characteristics. CHI and the cross-linking agent tripolyphosphate-sodium(TPP) can form biocompatible spheres that can be efficiently employed in bioactivesdelivery. Hence, we designed and developed chitosan-based nanocarriers for RSVencapsulation to overcome this physicochemical and pharmacokinetic limitations. Ourresults included the design of colloidal NPs of CHI-TPP obtained by ionic gelation. Theparticle size distribution and the Z-potential were recorded by dynamic light scattering(DLS). The peak of the majority population was located at 144 nm and the obtained value ofthe Z-potential was 21±4 mV, indicating that the suspension of the NPs is relatively stable.We registered CHI-RSV interactions by fluorescence quenching. In addition, the protectiveeffect of CHI against UV-induced photodegradation of RSV was determined. Also, weobtained TEM images for NPs size and shape characterization. They were rounded.Biological studies performed in human retinal pigment epithelial cells (ARPE-19 cells,ATCC) revealed that both free CHI and NPs were not cytotoxic. Also, uptake andintracellular incorporation was monitored through fluorescence microscopy. Finally, RSVloading- efficiency of the NPs was measured using spectrophotometry. RSVnanoencapsulation could be a potential carrier for the bioactive, opening the possibility of itsapplication for ophthalmic uses.