Osmotic stress and human amnion aquaporins

DI PAOLA, M.; ERLEJMAN, A.G; DOROSHNKO, B; DAMIANO, A.; FERNANDEZ, N

Rosario

Congreso; Reunión Anual Sociedad Argentina de Fisiología; 2019

Sociedad Argentina de Fisiología

INTRODUCTION: Amniotic fluid (AF) is essential for normal fetal growth. Aquaporins (AQPs) may have a key role in the AF volume homeostasis. Therefore, altered expression of these proteins might be associated with pathologies as oligo and polyhydramnios. However, the etiology of these syndromes remains unknown. Recently, we demonstrated that AQPs facilitate water transport across human amnion, being the AQP1 the most important contributor.OBJETIVE: To study the effect of an osmotic stress on the expression and regulation of AQPs in amniotic cell line WISH.METHODS: WISH cell line were cultured in hypo (150 mOsm) and hyperosmolar (400 mOsm) conditions. AQP1, AQP3 and AQP9 expressions were examined. Tetraethylammonium (TEA) and CuSO4 were used to block AQP1 and AQP3, respectively. Cell viability and were studied by the MTT and LDH assays. Cell swelling was studied with phase contrast microscope and Image-J software. Nf-kB and I-kB expression were assessed in nuclear and cytosolic fractions. RESULTS: AQP1 and AQP9 significantly decreased (n=6; p