MICE PATERNAL ALCOHOL EXPOSURE AFFECTS EPIGENETICS MARKS ON SPERM ALTERING THE TESTIS STRUCTURE FROM ITS OFFSPRING

GOTFRYD L; ROMANATO M; STINSON MG; FONTANA VA; CAMBIASSO MY; CALVO JC

Córdoba

Congreso; IX INTERNATIONAL MEETING of the Latin American Society for Biomedical Research on Alcoholism (LASBRA); 2019

Latin American Society for Biomedical Research on Alcoholism (LASBRA)

Previously, we observed that male alcohol consumption increased the sperm rate of decondensation, delayed embryo differentiation by deregulating peri-implantation events and altering embryo trophoblast and inner cell mass morphology in vitro. This study evaluates the effect of paternal alcohol consumption on spermatozoa and testis and its effect on male offspring. CF-1 male mice were exposed (treated group, T) or not (control group, C) to 15% (v/v) ethanol in drinking water ad libitum for 15 days. Sperm from epididymal cauda were obtained by swim-out to determine sperm oxidative stress with the CellROX Green Flow Cytometry Assay Kit and epigenetic marks of histone for immunocytochemistry. Testicles were weighted and the DNA fragmentation was analyzed by TUNEL assay on both groups. Males from control and treated groups were mated with non-treated CF-1 female mice in a ratio 1:1. Sperm from cauda of adult male mice of the offspring (F1) were obtained by swim-out to determine sperm parameters and head decondensation. Testicles of F1 mice were weighted and analyzed histologically. Male alcohol consumption did not alter testicular weight but increased sperm oxidative stress (fluorescence intensity: 2,2±0,2 n=5 C vs 3,0±0,2 n=4 T, p