Uso potencial de baicalina y quitosano en el diseño de nanomedicina curativa para heridas de piel

PEREZ OE; ALAIMO A; DI SANTO MC

Jornada; II Jornadas de Jóvenes Bionanocientíficxs.; 2020

IQUIBICEN-CONICET, CIBION-CONICET, INS-UNSAM e Instituto Leloir

Baicalin (BAI) is a flavonoid of natural origin, obtained from the roots of Scutellaria baicalensis. Itsmedicinal use has been part of traditional Chinese forms of therapy for ancient times. It has antioxidant,antimicrobial and antitumor effects [1]. BAI is a labile compound at alkaline pH. The healing of complexwounds involves the combined action of different types of cells like fibroblasts, keratinocytes, epidermaland endothelial cells that act to promote the regeneration of new tissue [2]. Chitosan (CH) is a naturallyoccurring biopolymer that, like BAI, has proven effects in improving wound healing [3]. In previous workswe demonstrated the beneficial effect of Argentine CH in the wound healing process through theimprovement of cellular responses induced by PDGF (Platelet derived-growth factor) [4]. In this work,using cell cultures of human keratinocytes (HaCaT cells) and mouse fibroblasts (3T3-L1), we aim to studythe effect of these components on cell viability depending on the preparation and concentration. For this,the crystal violet technique and the analysis of microscopic images were performed. The results showthat BAI at low concentrations (5-25 μM) increases the viability of fibroblasts in the presence of FetalBovine Serum (FBS) and CH has a beneficial effect at a range of 50-1000 μg/mL. However, at highconcentrations of BAI (50-100 μM), a cytotoxic effect was observed by both spectrophotometric andmicroscopic analysis. On the other hand, at any BAI concentrations, the viability is compromised inkeratinocytes in the absence of FBS. Under 10 % FBS supplementation, the pH of the culture medium isclose to neutrality, while the culture medium in the absence of FBS has a pH range of 8-8.5. These resultsdemonstrate that pH conditions are critical for the BAI-CH nanoencapsulation success. We conclude thatby controlling the solution pH and the concentration of the bioactive compound, BAI could be efficientlynanoencapsulated in chitosan matrices for accelerating the wound repair process.