IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Trophoblastic cell invasion is induced by leptin involving Catenin/Wnt pathway
Autor/es:
RODRIGO RIEDEL; MARIANA JAIME; CECILIA L. VARONE; TAMARA FERNÁNDEZ ARANGUREN; AYELÉN TORO; JULIETA L. MAYMÓ; MALENA SCHANTON; BERNARDO MASKIN
Reunión:
Congreso; International Federation of Placenta Associations IFPA; 2019
Resumen:
IntroductionLeptin is a hormone synthetized within placenta, where it is known to exert a paracrine and autocrine actions. In addition, it has been shown to play a significant role at the early stages of pregnancy as it modulates critical processes such as migration and invasion of trophoblastic cells.ObjectiveThis work is intended to examine the mechanisms involved in the effect of leptin regulation of trophoblast invasion in placenta, focused on the β-Catenin/Wnt pathway MethodsSwan-71 cells, a first trimester trophoblastic human cell line and human term placental explants were used , Swan-71 cells and placental explants were treated with different concentrations (50, 100 and 250 ng/ml) of recombinant leptin during 48 h (Swan-71cells) or 20 h (placenta explants). Metalloproteinases 2 and 9 (MMP-2 and MMP9) were assessed by qRT-PCR and zymography. -Catenin expression was determined by Western-Blot and immunofluorescence analysis. The action of XAV939 a β-Catenin/Wnt inhibitor was also used. Invasion experiments using trans-wells covered with Matrigel were also performed.ResultsWe determined that leptin treatment increased MMP-2 and MMP-9 levels of mRNA and enhanced their activity. Moreover leptin treatment enhanced invasion of trophoblastic cells. ConclusionsLeptin increases invasiveness of trophoblastic cells by enhancing MMP2 and MMP9 expression and activity.Moreover leptin increases β-CateninTherefore; -Catenin/Wnt pathway seems to be one of the signalling pathways involved in increasing the invasiveness of trophoblastic cells