IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Embryo quality modulates the generation of an inflammatory microenvironment by decidualized cells
Autor/es:
FERNANDEZ L., GRASSO E., SOCZEWSKI E., GORI S., CALO G.§, HAUK V., IRIGOYEN M., MARTINEZ G., PÉREZ LEIRÓS C. AND RAMHORST R.
Lugar:
Copehangue
Reunión:
Congreso; European Society or Humand Reproduction and Embriology; 2020
Resumen:
Study question: Could soluble factors secrete by embryos (embryo conditioned media or ECM) control the inflammatory response during the peri implantation period according to embryo quality? Summary answer: Decidualized cells increased the expression/production of inflammatory cytokines as wells as neutrophils migration and activation in response to ECM from impaired development (ID) embryos.What is known already: The decidualization program in humans starts on each menstrual cycle and implies not only phenotypical changes on the endometrial stromal cells, but also in their secretory profile. This secretome includes pro-implantatory factors as well as pro and anti-inflammatory cytokines. Despite embryo implantation is associated to a sterile inflammatory response, it should be later controlled in favor of a tolerogenic microenvironment. In this sense, decidualized cells display the ability to change their secretome according to the quality of the embryo. Study design, size, duration: Human endometrial stromal cell line (HESC) was decidualized with medroxiprogesterona and dbcAMP during 8 days. Then, decidualized HESC cells were stimulated with human embryo condition media (ECM) obtained from developing blastocysts (normal development or ND) or embryo-stage arrested ones (impaired development or ID). Non decidualized cells were used as control. Participants/materials, setting, methods: Embryo conditioned media (ECM) were recovered from 5 days individually cultured embryos obtained from IVF/ICSI and classified as normal or impaired development (ND/ID) according to Istanbul consensus. Neutrophils were obtained from peripheral blood from healthy donors. Cytokine expression/production was evaluated by RT qPCR/flow cytometry/ELISA. Caspase 1 activity was measured using Flica probe. Neutrophils migration towards HESC supernatants was evaluated using a transwell system. ROS production was assessed by adlaf probe. MMPs activity was evaluated by gelatin zymography.Main results and the role of chance: We observed that ID-ECM stimulation increased caspase¬-1 activation and IL 1β production by decidualized cells, while ND-ECM reduced IL 1β production (p