Periodontal Condition and Trophoblastic Function in Pregnant Women

DOGA L, GLIOSCA L, MASSONE C, MOLGATINI S,; PEREZ LEIROS C; DERAMO L,; RAMHORST R ; HAUK V, CALO G, MERECH F, LARA B, *, ; SQUASSI A

Washington DC

Congreso; 2020 IADR/AADR/CADR Meeting; 2020

Objectives: To explore the mechanisms underlying placental defects associated with the presence of periodontopathic bacteria in the mouthMethods: Periodontal examination was performed in 11 pregnant women at 16-20 weeks of gestation (bleeding on probing-BP, probing depth-PD and clinical attachment level-CAL) by calibrated dentists. Subgingival biofilm samples were collected by placing sterile paper points into periodontal pockets (4 sites per patient). Samples were homogenized, aliquoted and preserved at -20°C until its use in functional trophoblastic /neutrophil assays and qPCR determinations of periodontopathic bacteria. Total genomic DNA was obtained using affinity columns (Presto TM Mini gDNA Bacteria kit Geneaid) and quantified before bacteria detection by qPCR using specific primers. Neutrophils were isolated from peripheral blood of healthy donors by Ficoll-Dextran and cultured in vitro with biofilm samples (dilution range 1:2-1:10) for 45 min before reactive oxygen species (ROS) formation was assessed by flow cytometry with a fluorescent probe. In parallel, biofilm samples were added to first trimester trophoblast cell line (Swan 71) monolayers for 18 h and then migration assessed in 24 well plates by wound healing assays.Results: 1779 periodontal sites were evaluated (BP = 13.2%). The periodontal status indicators were (x +/- SE): BP: 0.13 +/- 0.01; PD: 2.20 +/- 0.02; CAL: 2.21 +/- 0.02. 53% of sites had PDS> 3mm and 13.71% PD> 5mm. All patients presented BP in> 1 site and only 4 presented BP in