IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Structural and functional analysis of TcCARP1 and TcCARP3 in Trypanosoma cruzi
Autor/es:
IVAN CAMPEOTTO; MARTIN M EDREIRA; GABRIEL FERRI
Lugar:
Mar del Plata
Reunión:
Congreso; XXXI Reunion Anual Sociedad Argentina de Protozoologia; 2019
Resumen:
Chagas disease is a chronic life-threatening disease caused by the protozoan parasite Trypanosoma cruzi, which represents a significant health burden in Latin America. For a targeted therapy of this disease, new antiparasitic drugs should be specifically directed against essential pathways in the parasite life cycle. Among these potential targets are signal transduction pathways, which have remained largely unexplored in trypanosome species. Of special interest is cAMP-mediated signaling, since cAMP has been shown to play critical roles in the life cycle of T. cruzi. The present research focuses on the identification and characterization of TcCARP1 and TcCARP3, cAMP Related Proteins (CARPs) in T. cruzi recently described by Gould et al, while investigating the mechanism of resistance of the parasite to the CdpA, an inhibitor of phosphodiesterases. Within CARPs, it has been shown that TcCARP1 and TcCARP3 presented a significant increase in both gene expression and protein translation during host cell invasion, only in virulent strains parasite. The aim of this research was to elucidate CARP mediated signaling in T. cruzi, through protein-protein studies, subcellular localization and oxidative stress assays; providing structural and functional analysis of CARPs proteins, not only to increase our knowledge about T. cruzi biology but also to target CARPs for the design and development of novel therapeutic agents against Chagas disease.