IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
The SUMO conjugation capability of Akt affects the expression of the pluripotency transcription factor Nanog in mouse embryonic stem cells
Autor/es:
MARCOS GABRIEL FRANCIA; MARIA VICTORIA PETRONE; ALEJANDRA GUBERMAN; CAMILA VÁZQUEZ ECHEGARAY; AYELEN TORO; MARTIN STORTZ; VALERIA LEVI
Lugar:
buenos aires
Reunión:
Congreso; Xth Meeting of the Latin American Society for Developmental Biology; 2019
Institución organizadora:
LASDB
Resumen:
Embryonic stem cells (ESCs) are an excellent model to study molecular mechanisms involved in early development. The transcription factors (TFs) Nanog, Oct4 and Sox2 promote pluripotency and repress differentiation. In cell culture, pluripotency is classically preserved by serum and LIF. This cytokine turns on multiple signaling pathways, among them, the PI3K/AKT pathway, which is crucial for self-renewal and pluripotency maintenance. Modification of Akt by SUMO conjugation regulates its activity in different cell lines and cancer, however, its role in ESCs has not been studied yet.In this work we studied the effect of the SUMO conjugation of Akt1 on Nanog gene regulation. We used Akt1 variants with different capability of being SUMOylated. We evaluated their effect on Nanog gene promoter and on the endogenous Nanog protein. We found that those variants that can be SUMOylated induced Nanog gene, however, this effect was completely absent with those variants in which SUMO conjugation is diminished. To study the molecular mechanism involved, we analyzed the effect of putative mediators? downregulation by shRNA. We found that the SUMO conjugation capability of Akt affects the expression of the pluripotency TF Nanog. Our results suggest that neither Oct4, Sox2 nor Nanog itself are responsible for the effect produced by the Akt1 variants on Nanog promoter. We are conducting further research to unravel the molecular mechanism involved, focusing on a strong candidate, the TF Tbx3.