IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
INFLUENCE OF THE CARBON SOURCE QUALITY ON AGE-RELATED PROCESSES IN YEAST
Autor/es:
GUILLEN-VALENCIA JENNIFFER; BERMÚDEZ-MORETTI, MARIANA; MUÑOZ SEBASTIAN ANIBAL; GULIAS JUAN FACUNDO; CORREA-GARCÍA, SUSANA RAQUEL
Lugar:
Paraná
Reunión:
Congreso; LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2018
Institución organizadora:
SAIB
Resumen:
Glucose is the primary source of carbon and energy for yeast. Glucose induces glycolytic enzymes and represses the utilization of alternative carbon sources. The Snf1 kinase is a key component of the cellular response to fluctuations in the levels and quality of the carbon source. Its activity is stimulated by glucose limitation, and induces genes that regulate the metabolism of alternative carbon sources, gluconeogenesis and respiration. Taking into account that one of the best known interventions to prolong lifespan is the caloric restriction and that Snf1 is considered a global energy regulator, the aim of this work was to study the participation of Snf1 in cellular longevity. We used strains deficient in the kinases Snf1 and Tor1 and minimal media with carbon sources of different quality. We found that mitochondrial activity of cells lacking these two kinases is higher than in wild type cells grown on non-fermentable carbon sources. All mutant strains grown on a respiratory carbon source are tolerant to oxidative stress. Cells lacking Snf1 and/or Tor1 from a fermentable carbon source at stationary phase are more tolerant to oxidative stress than wild type cells. We found that wild type cells from a strictly respiratory carbon source present a longer chronological lifespan than those from a fermentable carbon source. On caloric restriction growth media, strain is more long-lived than wild type and strains. On restricted carbon sources, there is more autophagy in wild type and cells than in cells. These results indicated that the age-related process, autophagy, is inversely associated with lifespan.