Decidual macrophages and trophoblast cell phenotype and function are regulated by VIP in human first trimester placenta

RUHUL CHOUDHURY; JOHN APLIN; DAIANA VOTA,; ROSANNA RAMHORST; DANIEL PAPARINI; GUILLERMINA CALO; CLAUDIA PÉREZ LEIROS

Mar del Plata

Congreso; Reunión Anual SAI; 2018

Deep placentation disorders are associated with impaired invasiveness of extravillous trophoblast cells (EVT) and vascular remodeling in a pro-inflammatory microenvironment. The vasoactive intestinal peptide (VIP) has anti-inflammatory, pro-secretory and vasodilating effects. However its effect on decidual macrophages (dMA) and pregnancy outcome is still unclear.Here we studied the role of VIP in human first trimester placenta as a regulator of dMA and EVT function.Positive selection with CD14-immunomagnetic beads was used to isolate dMA from human placental explants (5-9 weeks). Explants or dMA were cultured ±VIP (10-100 nM). Gene expression and protein secretion was studied by qPCR and BioPlex assay, respectively. Tubulogenesis assay was performed in uterine endothelial cells (EC) in the presence of supernatant from dMA. The first trimester trophoblast derived cell line, BeWo (Tb), was knocked-down for VIP (VIP-KD) with a specific siRNA and spiral artery (spA) remodelling was studied by immunostaining assays.VIP increased IL-10 (116±37 vs. 81±32 pg/ml; P