IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Dal81 and Uga3 transcription factors and stress response in Saccharomyces cerevisiae
Autor/es:
MUÑOZ SEBASTIAN ANIBAL; VALENCIA-GUILLEN JENNIFFER; GULIAS JUAN FACUNDO; BERMÚDEZ-MORETTI, MARIANA; MERCAU MARIANA; CORREA-GARCÍA, SUSANA RAQUEL
Lugar:
Paraná
Reunión:
Congreso; LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2018
Institución organizadora:
SOCIEDAD ARGENTINA DE INVESTIGACIÓN BIOQUÍMICA Y BIOLOGÍA MOLECULAR-SAIB
Resumen:
S cerevisiae is able to metabolize a wide variety of nitrogen compounds. For example, amino acids are used as nitrogen or carbon source, both in anabolic or catabolic processes. The -aminobutyric acid (GABA) is an amino acid widely distributed in nature and has several functions on different organisms. Particularly, yeast cells can use it as sole nitrogen source. GABA presence determines the activation of the pleiotropic (Dal81) and the specific (Uga3) transcription factors (TF), which induce the expression of the UGA genes. The proteins necessary for GABA catabolism are encoded by UGA4 (GABA specific permease), UGA1 (GABA transaminase) and UGA2 (succinate semialdehyde dehydrogenase). In addition, some authors have proposed that the activity of the GABA metabolic pathway affects cells tolerance to stress. In this study we analyzed the effect of TF involved in GABA genes regulation on stress response. We found that Uga3 has a very important role on tolerance against both heat shock and oxidative stresses. Dal81 is also important for cell survival after stress, although at a lesser extent. However, GABA induction of the genes regulated by Uga3 and Dal81 does not produce changes in cell growth after heat shock and oxidative stress. Early life stress can have positive impacts on longevity and during the normal aging process global protein synthesis generally declines with increased organismal age. However, UGA4 expression increases with the culture age independently on the presence of Uga3 and Dal81. These results suggested to us that Uga3 and Dal81 could have a physiological role on stress tolerance, besides GABA metabolism regulation.