AGING AND EXPRESSION OF PERMEASE GENES IN Saccharomyces cerevisiae

BIRENBAUM, JOAQUÍN; MUÑOZ SEBASTIAN ANIBAL; GULIAS JUAN FACUNDO; CORREA-GARCÍA, SUSANA RAQUEL; GUILLEN-VALENCIA JENNIFFER; BERMÚDEZ-MORETTI, MARIANA

Paraná

Congreso; LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2018

SAIB

Amino acids serve as precursors for synthesis of proteins and other metabolites and also as carbon and nitrogen sources. Yeast cells are able tointernalize amino acids since they possess permeases that facilitate their transport. Amino acid utilization is subjected to regulatory pathwayssuch as GAAC (General Amino Acid Control), TORC1 and amino acid sensor SPS. These pathways operate in parallel and in a coordinated wayto generate the correct transcriptional gene response to nutrient availability. The aim of this work was to study the expression levels of twopermeases during aging and to analyse if these nutrient-sensitive pathways are involved in their transcriptional regulation. In particular, wefocused on the Uga4 and Bap2 permeases, responsible for the uptake of the gamma-aminobutyric acid (GABA) and the branched-chain aminoacids, respectively. We used S. cerevisiae mutant strains grown under two dietary conditions (with and without all amino acids), andtranscriptional activity was measured. We found that in wild type cells UGA4 expression increases along with aging in the growth conditionsused, even in the absence of its inducer, GABA, and the GAAC pathway is partially responsible in maintaining these elevated levels. Incontrast, BAP2 expression does not vary with aging in wild type cells grown without amino acids, but it is higher in cells grown with amino acidsat the beginning of the aging process and decreases after cells reach stationary phase. This behavior depends on GAAC. Further research must bedone to understand the differential transcriptional profiles of the permeases studied and the influence they have on lifespan