Resveratrol confers protection against oxidative stress and mitochondrial damage in an in vitro model of Age-Related Macular Degeneration

AGUSTINA ALAIMO; OSCAR E PÉREZ; FLORENCIA BUOSI; GUADALUPE GARCÍA LINARES

Buenos Aires

Congreso; Fronteras en Biociencia 3; 2018

Instituto de Investigación en Biomedicina de Buenos Aires- Partner of the Max Planck Society

Age-related macular degeneration (AMD) is a late-onset neurodegenerative retinal disease and the leading cause of central vision loss in the elderly. Degeneration of retinal pigment epithelial (RPE) cells is a crucial causative factor responsible for the onset and progression of AMD. The toxic fluorophore N-retinyl-N-retinylidene ethanolamine (A2E), a major lipofuscin component, accumulate in RPE cells with age. Phytochemicals with both antioxidant and anti-inflammatory properties may have potential role in the prevention and treatment of this age-related ocular disease. Particularly, there is an increased interest in the therapeutic effects of Resveratrol (RSV). Mitochondria frequently change their shapes by fusion and fission and these morphological dynamics play important roles in mitochondrial function as well as cell death.The study intended to explore the protective effectiveness of RSV against A2E-induced toxicity in RPE cells. For this purpose, human ARPE-19 cell line were cultured and exposed to 25 μM A2E in the absence or presence of RSV (10 and 25 μM). The following parameters were studied: mitochondrial metabolic activity as cell viability indicator (MTT assay), generation of mitochondrial reactive oxygen species (ROS) (MitoSOX fluorescent probe), mitochondrial shape-changes quantification (MitoTracker Red CMXRos fluorescent probe) and, apoptosis (DAPI, fluorescent probe). The obtained results showed that pretreatment with RSV significantly attenuated A2E-induced viability decreases (10μM: 14%; 25 μM: 15%; p