FXYD5/DYS is associated with high risk of recurrence in endometrial cancer (EC) and modulates cell migration and NF-κB activation in EC cells

ROSSO, MARINA; ELIZALDE, PATRICIA V.; VÁZQUEZ-LEVIN, MÓNICA; MERCOGLIANO, MARÍA FLORENCIA; LAPYCKYJ, LARA; BESSO, MARÍA JOSÉ; SCHILLACI, ROXANA; MATOS, MARÍA LAURA

Mar del Plata

Congreso; LXIII Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2018

Sociedad Argentina de Investigación Clínica (SAIC)

Endometrial cancer (EC) is the 6th most common cancer in women worldwide. Endometrioid Endometrial Carcinomas (EEC) are ~80% of EC cases. Myometrial invasion (MI) is a key event in EC dissemination and deep MI is critical to define EC recurrence risk and associated with poor prognosis. Epithelial Cadherin (Ecad)-mediated cell-cell adhesion is altered, and Epithelial to Mesenchymal Transition (EMT)-related events occur during tumor progression in EEC, but the molecular basis are not fully known. We have identified a novel E-cadherin splice variant (Ecadvar) mRNA that induces an EMT phenotype in a breast cancer model. The present study aims to evaluate Ecadvar mRNA expression levels in EEC.Materials & Methods: QRT-PCR assays were run to quantify Ecadvar expression relative to Ecadwt in (A) In vitro studies with Hec1a cells (A1) stably transfected with ETV5 transcription factor, found in the invasive front of EC, (A2) treated with TGF-β1, a cytokine that induces EMT, (A3) transiently transfected with Ecad siRNA to downregulate the wild type Ecad. (B) Tissue endometrial biopsies from EC patients and controls.Results: (A1) Hec1a-ETV5 cells showed higher (p