Comparison between water microbial communities upstream and downstream a water treatment plant in Moreno district, province of Buenos Aires

DAISY BERNAL REY; LOPEZ, NANCY I.; FACUNDO J. ALAMSQUÉ; RENATA MENENDEZ; LAURA J. RAIGER IUSTMAN; JOSE G. IBARRA; MARIA DEL CARMEN RIOS

San Luis

Congreso; XIII Congreso Argentino de Microbiologia General SAMIGE 2018; 2018

SAMIGE

In Cuartel V, district of Moreno, Province of Buenos Aires, there is an industrial waste treatment plant (WTP) placed near a natural stream. This water course is also surrounded by horticulture farms and urban settles. Local inhabitants have reported recurrent WTP over-flooding that have affected the water course and some adjacent farms, thus water contamination became an important social and economic problem. The aim of this work was to analyze the microbial community in a reference site located upstream the WTP (R) and a site located downstream the WTP (DS) sites to find indicators of the biological conditions of the system. Water samples were collected on 2017 July 19th (winter) and December 12nd (spring) in both DS and R sites. After collection, samples were conserved at room temperature until processing within 24 h. Total DNA was extracted using the DNeasy® PowerWater® kit (QIAGEN) and 16S rRNA gene V1-V3 region was sequenced by Illumina my-seq (Mr DNA-Texas, USA). Community diversity was analyzed using QIIME platform. Diversity index Shanonn (H) and Simpson (D) showed that in winter samples, DS presented a significant higher diversity compared with R (D: 0.985 and 0.954, H: 7,977 and 6,620 respectively p