Conditioning of myeloid regulatory cells by the decidualization process

GORI MS, SOCZEWSKI EV, GRASSO E, FERNÁNDEZ L, BLEJER J, GRASSI BASSINO A, PÉREZ LEIRÓS C, SALAMONE G, RAMHORST R.

Mar del Plata

Congreso; Reunión Anual Conjunta SAI-SAIC-SAFIS; 2018

SAI-SAIC-SAFIS

The decidualization process involves phenotypic and functional changes on endometrial cells and the modulation of immunoregulatory mediators that may condition maternal leukocytes, as dendritic cells (DC) and macrophages, to a regulatory fate. In fact, Myeloid regulatory cells (Mregs) have recently been focus of attention since their presence at the materno-fetal interface and their potential role as immune-suppressors. Here, we focus on the impact of decidualization process in conditioning CD14+ cells to Mregs subsets. For this, we collected conditioned media (CM) of Human endometrial stromal cell line (HESC) decidualized with MPA+dbcAMP for 8 days (Dec-CM) or not (Non-dec CM). Then, isolated monocytes from peripheral blood mononuclear cells from healthy donors were cultured with rhGM-CSF+rhIL-4 for 5 days in the absence/presence of CM. We observed that Dec-CM was able to inhibit CD14+ cells differentiation to immature DC, in a concentration-dependent manner, evidenced by the persistence of high-proportions of CD14+CD1a- cells after 2 and 5 days of culture (p