IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Post-transcriptional regulation and mRNA localization of PKA subunits in Saccharomyces cerevisiae
Autor/es:
BARDECI, N; FERNÁNDEZ NÚÑEZ, L., ROSSI, S., BLUMENTHAL, D., MORENO, S.; PAUTASSO C; PORTELA, P; CAÑONERO, L; GALELLO, F
Lugar:
Buenos Aires
Reunión:
Congreso; LIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular, Reunión Conjunta de Sociedades de Biociencias; 2017
Institución organizadora:
SAIB
Resumen:
In the yeast Saccharomyces cerevisiae, the protein kinase A (PKA)controls a variety of cellular events as a consequence of particularstimulus. The specificity of the cAMP signal transduction is maintainedby several levels of control acting simultaneously. Our aim isto study the regulation of PKA subunits expression as one of suchcontrol levels, which is directed by the promoter?s activity and themRNA stability and localization. The balance among transcription,degradation and translation of individual mRNAs can be differentiallyregulated in response to environmental conditions such as heatstress. PKA holoenzyme from S.cerevisiae is composed by a dimerof regulatory subunit encoded by BCY1 gene and two catalytic subunitsencoded by TPK1, TPK2 and TPK3 genes. Previously we haveshown that the promoter of each PKA subunit is differentially regulatedduring heat shock, particularly TPK1 promoter is the only promoteractivated. Here we demonstrate that BCY1 mRNA half-life islonger than TPK1 mRNA half-life and that TPK1 and BCY1 mRNAsare stabilized upon heat stress, as shown by Northern blot analysis.In contrast, Western blot analysis showed that Tpk1 protein abundanceis not increased during heat shock. We also demonstrate thatTPKs and BCY1 promoter sequences can influence the levels ofmRNAs abundance. TPK1 mRNA localization was analyzed in vivousing the m-TAG methodology, showing the formation of ribonucleoproteinfoci upon heat shock. Overall, the results suggest that inresponse to heat shock stress PKA subunit mRNAs increase theirabundance and are concentrated in foci allowing a faster responseonce the environment is again favorable.Keywords: PKA, mRNA, heat stress, localization, stability