Arginine and cysteine funcionalizated Fe3O4 NPs, a superparamagnetic and monodisperse system for bacteria capture applications

EDUARDO CORTON; ALBERT SAAVEDRA; FEDERICO FIGUEREDO; VIRGINIA DIZ

Bariloche

Simposio; XVII Encuentro de Superficies y Materiales Nanoestructurados; 2017

Instituto Balseiro (CNEA)

We demonstrate a simple two-step synthesis and functionalization process for thepreparation of iron oxide nanoparticle (NP) aqueous colloids using arginine andcysteine, denominated Fe3O4@Arg and Fe3O4@Cys, respectively. The processesinvolve co-precipitation in presence of oxalate, followed by amino acid substitution. Thesignals obtained by Fourier transformed infrared spectrometry (FT-IR) show thepresence of functional groups belonging to the amino acids that stabilize the surface ofthe magnetite NPs. The images of field emission scanning electron microscopy (FE-SEM) showed homogeneous distribution of particle sizes for all systems synthesized;11.4 ± 2.3 and 12.3 ± 2.5 nm for Fe3O4@Arg and Fe3O4@Cys, respectively.Magnetization, zeta potential and energy dispersive spectroscopy (EDS) studies allowus to further characterize the obtained amino acid modified NPs. According to theresults reported so far, and to our best knowledge, detailed FT-IR signals and zetapotential in a broad pH range are show for the first time for oxalate coated Fe3O4 NPs.We also show that such a simple one-step functionalization process can be useful in avariety of applications, where to remove or immobilize non-specifically bacteria from aliquid media is required. Capture experiments of Pseudomonas putida bacterial strainwith both systems, Fe3O4@Arg and Fe3O4@Cys showed a high level of efficiency;the effect of pH was also demonstrated.