Relevance of the Blastocyst Conditioned Media on immunotolerance: focus on the control of the inflammatory response.

E. GRASSO, E. SOCZEWSKI, D. PAPARINI, L. FERNÁNDEZ, L. GALLINO, G. MARTINEZ, M. IRIGOYEN, C. RUHLMANN. C. PÉREZ LEIRÓS, R. RAMHORST

Puerto Varas

Conferencia; Latin American Society for Maternal Fetal Interaction.; 2017

Implantation is associated with a sterile inflammatory response that should be later controlled to a tolerogenic microenvironment by maternal and blastocyst-derived factors. At decidualization, cells undergo reticular stress and unfolded protein response, allowing them to expand their endoplasmic reticulum and change their secretome increasing immunomodulators? production. Objective: We investigated whether human Blastocyst Conditioned Media (BCM) could control the initial inflammatory response during the implantation period. Methods: Human endometrial stromal cell line (HESC) were decidualized or not with medroxiprogesterone+dbcAMP. Then, HESC cells were treated with human BCM for 24 h. Gene and protein expression was evaluated by PCR or FACS respectively. An in vitro implantation model based on co-culture of blastocyst-like spheroids (BLS) from Swan-71 trophoblast cells line over decidualized-HESC cells and a transwell migration system for Tregs recruitment were used to evaluate decidual functionality. Results: We observed (p