VIP CONTRIBUTION TO THE SELECTIVE RECRUITMENT OF TREGS DURING THE PERI-IMPLANTATION PERIOD

GRASSO ESTEBAN; WASCHEK JAMES; GALLINO LUCILA; VOTA DAIANA; RAMHORST ROSANNA; HAUK VANESA; PEREZ LEIROS CLAUDIA

Buenos Aires

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

Decidualization process involves phenotypic and functionalchanges on endometrial cells and the modulation of different me-diators such as the secretion of the vasoactive intestinal peptide(VIP). This neuropeptide is a key regulator of the immunetolerancecapable of inducing Tregs towards alloantigens which are crucialfor a successful implantation and the pregnancy maintenance. VIPdeficient(+/-) pregnant mice have reduced litter size and increasedinter-gestations period.Here, we evaluated VIP contribution to the Tregs? recruitmentduring the peri-implantation period. We used two approaches:VIP(+/-) and Foxp3-knock-in-GFP females either pregnant or in es-trous. Foxp3 expression by RT-PCR was not detectable in VIP(+/-)mice uterus, in contrast to Foxp3-GFP mice, confirmed by FACSanalysis. This reduction was accompanied with a higher expressionof RORγt and a decrease in IL-10 (p