IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
FIBRE MICROPARTICLES OBTAINED FROM JAPANESE PLUM (PRUNUS SALICINA) HAVE ANTIOXIDANT ACTIVITY IN HUMAN CELLS
Autor/es:
CAMISAY, M.F.; ERLEJMAN, A.G.; DE LEO, S.A; ROJAS, A.M.; GARCIA, G.; BASANTA, M. F.
Lugar:
Buenos Aires
Reunión:
Congreso; LXII Reunion Anual de la Sociedad Argentina de investigación Clínica (SAIC); 2017
Institución organizadora:
Reunión Conjunta de Sociedades de BioCiencias
Resumen:
Japanese plums are largely grown for fresh consumption. Plums discarded by the food industry can be used as an important source of bioactive compounds such as antioxidant polyphenols as well as dietary fibres like pectins. Polyphenols are habitually co-extractedwith fibres. Certain polyphenols can exert cytoprotective actions in the gastrointestinal tract interfering with the oxidative stress implicated in inflammatory disorders. Fibre microparticles (MPCs) obtained through ethanolic extraction from plums retained polyphenolic compounds, mainly pentameric proanthocyanidins (170?200mg/100gMPCs) and smaller amounts of anthocyanins and flavonoids. The aim of this work was to biologically evaluate in vitro the polyphenols extracted from plum MPCs in relation to their capability to protect colorectal epithelium (Caco-2) human cells and embryonic kidney(HEK293) cells against the oxidative stress induced by tert-butylhydroperoxide (tBOOH), as well as in their cytotoxicity. Cellular oxidation induced by t-BOOH was evaluated through the dichlorofluorescein (DCF) oxidation. When Caco-2 cells were co-incubated with 0.5 to 10 μg/mL, the tBOOH induced oxidative stress was significantlyreduced (p