IQUIBICEN   23947
INSTITUTO DE QUIMICA BIOLOGICA DE LA FACULTAD DE CIENCIAS EXACTAS Y NATURALES
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Nano-delivery for drugs with antiviral properties based on BSA as a carrier
Autor/es:
CASTAÑEDA CATAÑA, MAYRA ALEJANDRA; PÉREZ OSCAR E.; MARTÍNEZ ,KARINA D; DODES TRAIAN, MARTÍN; SEPÚLVEDA, CLAUDIA S.
Lugar:
Porto
Reunión:
Conferencia; 31St International Conference of Antiviral Research; 2018
Institución organizadora:
ISAR International Society of Antiviral Research
Resumen:
The emergence of antiviral drug-resistant mutants is the most important issue in current antiviral therapy. An ideal therapeutic target to prevent drug-resistance development is represented by host factors that are crucial for the viral life cycle. Given that viruses are obligate parasites, several host factors that are crucial for viral replication also represent antiviral therapeutic targets, referred to as the ?cell-based approach?.Recent efforts have focused on finding and characterizing cellular metabolic inhibitors as broad-spectrum antivirals for targeting with minimum cytotoxicity.MPA is a non-nucleoside, non-competitive inhibitor of inosine monophosphate dehydrogenase (IMPDH). It is well known by its effects as immunosuppressive and against multiplication of several viruses; this inhibition was highly reversed by exogenous guanosine addition, indicating that antiviral activity is effectively associated with GTP depletion through IMPDH blockade.Albumin is emerging as a versatile protein carrier for drug targeting and for improving the pharmacokinetic profile of peptide or protein-based drugs. Principally, three drug delivery technologies can be distinguished: coupling of low-molecular weight drugs to exogenous or endogenous albumin, conjugation with bioactive proteins and encapsulation of drugs into albumin nanoparticles.Using the bottom-up and salvation methods we obtained nanoparticles around 10 nm and 100 to 200 nm respectively, determinate by scanning electron microscopy and Zeta-sizer Nano-Zs particle analyzer. Primary the particles were evaluated for their cytotoxic action in cultures of different cell lines where has been shown to be nontoxic, according to MTT method using standard concentrations. The preliminary results showed that this type of albumin carriers may be apt for used in the antiviral drug delivery.