Antiadipogenic effect of the antioxidant N-acetylcysteine on 3T3-L1 adipocytes

A PIERALISI; MATÍAS GABRIELLI; MAURICIO DE MARZI; CLAUDIA N. MARTINI; ROMINA MITAROTONDA; LILIANA GUERRA; DANIELA SOTO; MARIA DEL C VILA; JUAN CARLOS CALVO

Buenos Aires

Congreso; Reunion Conjunta de Sociedades de Biociencia; 2017

Reports about antioxidant effect on obesity are controversial. We showed that N-acetylcysteine (NAC) inhibits cellular lipid accumulation during 3T3-L1 adipocyte differentiation, through the inhibition of the expression of adipogenic transcription factor PPARγ and MAPK phosphorylation (Soto el al, Redox Rep 2016). Objective: Here we evaluated NAC effect on fully differentiated 3T3-L1 adipocytes (AC). Methods: Assays were conducted using AC, including different doses of NAC (0.01 to 5 mM) in culture media for 5 days and, we determined: NAC toxicity by MTT method, content of cellular triglycerides (Tg) and Oil-Red-O stained lipids (ORO), PPARγ protein expression by western blotting, mRNA levels of PPARγ and lipid protein perilipin (Pl) by RT-PCR. NAC content in nanoparticles was determined by Raggi method. Results: None of NAC doses were toxic. 5mM NAC treatment on AC (ACN) elicited a 60% decrease in cellular Tg content (1.22+0.09 gTg/g protein [AC] vs 0.49+0.03 gTg/g protein [ACN], p