ANTI-ADIPOGENIC EFFECT OF THE ANTIOXIDANT N-ACTEYLCYSTEINE ON 3T3-L1 ADIPOCYTES

PIERALISI A; GABRIELLI M; DE MARZI M; MARTINI C; MITAROTONDA R; GUERRA LN; SOTO D; VILA MC; CALVO JC

CABA

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedad Argentina de Investigaciones Clínicas

Reports about antioxidant effect on obesity are controversial. We showed that N-acetylcysteine (NAC) inhibits cellular lipid accumulation during 3T3-L1 adipocyte differentiation, through the inhibition of adipogenic transcription factors expression, such as PPARγ and, MAPK phosphorylation (Soto el al, Redox Rep 2016). Objectives: Here, we evaluated NAC effect on fully differentiated 3T3-L1 adipocytes (AC) and developed nanoparticles with NAC. Methods: Assays were conducted using AC, different doses of NAC (0.01 to 5 mM) were added to culture media for 5 days and, we determined: cellular content of triglycerides (Tg) and Oil-Red-O stained lipids (ORO), NAC toxicity by MTT assay, PPARγ protein expression by western blot assay, mRNA levels of PPARγ and lipid protein perilipin (Pl) by RT-qPCR analysis. NAC content in nanoparticles was determined by Raggi method. Results: Treatments with 0.01 to 5 mM NAC were not toxic on AC. 5mM NAC treatment on AC (ACN) elicited a 60% decrease in cellular Tg content (1.22+0.09 gTg/g protein [AC] vs 0.49+0.03 gTg/g protein [ACN], p